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. 2023 Feb 21;15(5):1357. doi: 10.3390/cancers15051357

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Heterogeneous expression of MSLN in solid tumors. (A) Calculation of the expression rate of MSLN in malignant cells of different solid tumors at the protein level with ImageJ. The percentage of MSLN-positive tumor cells among the total number of tumor cells was calculated by IHC. The result of IHC verified by two pathologists. (B) Calculation of the expression rate of MSLN in malignant cells of triple-negative breast cancer (GSE75688 and GSE118389), pancreatic cancer (GSE111672) and ovarian cancer (GSE118828) using single-cell sequencing data downloaded from the GEO database (https://www.ncbi.nlm.nih.gov/geo/ (accessed on 28 September 2022)). The percentage of tumor cells with MSLN expression >0 of the total number of tumor cells in the single-cell data was calculated.