FIG. 2.

Proliferation assay. (A) T-cell proliferation assay. LN-derived T cells were incubated with ConA (0 to 5 μg/ml) for 72 h, treated with [³H]thymidine for 6 h, harvested, and counted. (B) B-cell proliferation assay. Spleen-derived cells were incubated with LPS (0 to 25 μg/ml) for 72 h, further cultured with [³H]thymidine for 16 h, harvested, and counted. Experiments were repeated three times; representative results from one experiment are shown. Error bars, standard deviations. Symbols: +/+, wild-type; +/−, heterozygote; −/−, homozygote.