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. 2023 Jan 12;7(3):2200213. doi: 10.1002/gch2.202200213

Figure 4.

Figure 4

Dose‐dependent cytotoxicity, hemolytic side effect, and cell penetrating capability of ALA‐A2 peptide. a) ALA‐A2 dose–response relationship in A549 lung cancer and HT29 colon cancer cell lines. The cells were incubated with twofold dilutions of ALA‐A2 from 400 to 0 µm for 24 h, and then cell viability was measured by the MTT assay. b) RBC lysis assay for ALA‐A2 peptide toxicity testing. Human red blood cells were incubated with the peptides for 1 h. Triton X‐100 at a final concentration of 1% was used as a positive control. c) Confocal microscopy showed that FITC‐tagged ALA‐A2 peptide (green) could internalize into A549 lung cancer cells without altered membrane integrity, whereas Triton X‐100 (0.5% v/v; the positive control) could permeabilize cellular membrane allowing PI internalization (red). Hoechst 33342 (blue) was used for the nuclear counter staining. Scale bar: 50 µm.