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. 2001 Dec;21(24):8385–8397. doi: 10.1128/MCB.21.24.8385-8397.2001

FIG. 2.

FIG. 2

Effects of bombesin (Bomb) and NT on androgen-dependent PSA transcription. Stimulation of reporter gene activity in LNCaP and PC3 cells stably expressing AR [PC3(AR)2] or mock-transfected cells [PC3(M)] transfected with the reporter plasmids. (A) LNCaP cells were transiently transfected with the PSA (−630/+12 Luc) plasmid and then treated with either R1881 (1 nM), bombesin (100 nM), or NT (100 nM) for 24 h in medium with 10% charcoal-stripped FBS. (B) PC3(AR)2 and PC3(M) cells were transfected with the PSA (−630/+12 Luc) plasmid. After transfection, cells were treated with either R1881(1 nM), bombesin (100 nM), or NT (100 nM) for 24 h in medium containing 5% charcoal-stripped FBS and 50 μg of hygromycin per ml. (C) PC3(AR)2 and PC3(M) cells were transfected with ARE5-Luc and treated with either R1881 (1 nM), bombesin (100 nM), or NT (100 nM) for 24 h in 5% charcoal-stripped FBS. The results are taken from three independent experiments. The ratio of ARE luciferase to pRL-tk luciferase represents relative luciferase activity. The fold increase indicates the ratio of the normalized luciferase activities between the cells cultured without androgen and with androgen or bombesin. (D) Regulation of PSA secretion in LNCaP cells by bombesin or NT. The cells were incubated in the presence of bombesin or NT for 72 h.