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Inhibitory effect of flutamide on bombesin-induced cell proliferation. LNCaP cells were preincubated in the absence or presence of flutamide (Flu) for 30 min before the addition of R1881 (1 nM) or bombesin (Bomb) (100 nM) for 72 h under charcoal-stripped serum conditions. Then 20 μl of MTS was added to each well for 2 h at 37°C. After incubation, the absorbance or optical density at a wavelength of 490 nm (OD490nm) was read as described in Materials and Methods.
