FIG. 7.

Dominant-negative mutant of Src (SrcKR) and FAK (FRNK) blocks bombesin-induced AR pathway. LNCaP cells were cotransfected with the PSA-Luc reporter and a pRL-tk reporter plus SrcKR or FRNK or an empty vector and cultured in 5% charcoal-stripped FBS. The ratio of ARE luciferase to pRL-tk luciferase represents relative luciferase activity. The fold increase indicates the ratio of the normalized luciferase activities between the cells cultured without bombesin and with bombesin (Bomb). The results are taken from three independent experiments.