Figure 2.

Effects of GM-080 preparations and cell wall compartments in Th1 cytokines production of mouse splenocytes. We seeded 4 × 10⁵ mouse splenocytes into a 48-well plate, followed by treatment with viable GM-080 preparation with the indicated MOI or cell wall compartments purified from GM-080 (LTA and PGN) for 48 h. The culture supernatants were then harvested, and INF-γ (A–D) or IL-12 (E–H) production was determined using commercial ELISA kits. LPS (1 µg/mL) or ConA (1 µg/mL) was used as the positive control. N.D., not detected.