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. 2023 Feb 28;12(5):768. doi: 10.3390/cells12050768

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Effects of oral GM-080 on immune cell infiltration and eosinophilic cytokines in lungs of mice with AHR. BALF was collected 2 days after the final OVA challenge followed by centrifugation, and the cell pellet was collected. (A–D) Eosinophil (A), neutrophil (B), lymphocyte (C), and monocyte (D) infiltration was evaluated through microscopy after staining cells with Liu’s stain. (E,F) Eotaxin (E) or IL-5 (F) levels in BALF was determined using commercial ELISA kits. NC, nontreated control; H₂O, H₂O gavage; LP-L, GM-080 at 2 × 10⁶ CFU/mouse/day through gavage; LP-H, GM-080 at 1 × 10⁷ CFU/mouse/day through gavage. * p < 0.05, ** p < 0.01.