Table 4.
Comparison of the mRNA expression of oncogenic signaling related factors (KRAS, SOX2, MITF, BRAF, FOS, and STAT3), ECMs (COL4, COL6, FN, and αSMA), tight-junction related molecule (ZO-1) among 2D and 3D cultured 5 MM cell lines.
| WM266-4 | SM2-1 | A375 | MM418 | SK-mel-24 | ||
|---|---|---|---|---|---|---|
| KRAS | 2D | cont | (↑) | → | (↑) | (↑) |
| 3D | cont | ↑↑ | ↑ | ↑↑ | ↑↑ | |
| SOX2 | 2D | cont | ↓↓ | ↓↓ | ↓↓ | ↓↓ |
| 3D | cont | ↓↓ | → | ↓↓ | ↑↑ | |
| STAT3 | 2D | cont | (↓) | (↓) | → | ↑ |
| 3D | cont | ↓↓ | → | ↑↑ | ↑↑ | |
| BRAF | 2D | cont | → | → | (↑) | (↑) |
| 3D | cont | (↓) | → | ↑ | ↑ | |
| FOS | 2D | cont | → | → | ↑↑ | → |
| 3D | cont | → | ↓↓ | → | ↓↓ | |
| MITF | 2D | cont | → | ↓↓ | ↓↓ | ↓↓ |
| 3D | cont | → | ↓↓ | → | ↓↓ | |
| PCG1a | 2D | cont | ↓↓ | ↓↓ | ↓↓ | ↓↓ |
| 3D | cont | ↓↓ | ↓↓ | ↓↓ | ↓↓ | |
| COL4 | 2D | cont | ↓↓ | ↓↓ | ↓↓ | ↑↑ |
| 3D | cont | ↓↓ | ↓↓ | ↓↓ | ↓↓ | |
| COL6 | 2D | cont | ↓↓ | ↓↓ | ↓↓ | ↓↓ |
| 3D | cont | ↓↓ | → | ↓↓ | ↓↓ | |
| FN | 2D | cont | ↓↓ | ↓ | ↑ | ↓↓ |
| 3D | cont | → | ↓↓ | ↓↓ | ↓↓ | |
| aSMA | 2D | cont | ↓↓ | ↓↓ | ↓↓ | ↓ |
| 3D | cont | ↓↓ | ↓↓ | → | ↓ | |
| ZO1 | 2D | cont | ↓↓ | ↓↓ | ↓↓ | ↓↓ |
| 3D | cont | ↓↓ | → | ↓↓ | ↓ |
no significant change; →. upregulated; (↑) = relatively, ↑ < 0.05, ↑↑ < 0.01. downregulated; (↓) = relatively, ↓ < 0.05, ↓↓ < 0.01.