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. 2023 Feb 22;12(5):694. doi: 10.3390/cells12050694

Figure 10.

Figure 10

Transfection of miRNA-146a-5p inhibitor in FAβ1–42-treated astrocyte cells rescues expression of IRAK-1 and TRAF-6 but not IRAK-2. (A) qRT-PCR evaluation of performance of anti-miR-146a oligonucleotides (AMO-146a) to down-regulate expression of miRNA-146a-5p in astrocytes. (B) qRT-PCR evaluation of impact of AMO-146a transfection on expression of transcriptional targets of miRNA-146a-5p in FAβ1–42-astrocyte-treated cells. Cells were first transfected with AMO-146a or AMO-CTL (100 nM), then treated with FAβ1–42 peptides solutions (2 µM) before quantification of the relative expression of IRAK1/2 and TRAF-6 transcriptional targets of miRNA-146a-5p. Non-treated astrocytes (NT, no FAβ1–42 treatment) were used as additional control. Data are represented as mean ± SEM of one representative experiment performed twice in triplicate. Statistical comparisons between groups were made using a Student’s t-test. * p ≤ 0.05, ** p ≤ 0.01.