Table 2.

Specific primer sequences for engineered plasmids.

Construction	Name and Direction *	Sequence **
prrnBP1-gfpOVA	GFP-vector-FW	5′-CGGAATAACTCCCTATAATGCGCCACCACTTCTAGATTTAAGAAGGAGATATACATATGA-3′
	GFP-vector-RV	5′-AACGCTGTAAAACGGGCAATAATTGTTCAGCGCATGCACCATTCCTTGCGGCG-3′
	rrnB1-insert-FW	5′-CGCCGCAAGGAATGGTGCATGCGCTGAACAATTATTGCCCGTTTTACAGCGTT-3′
	rrnB1-insert-RV	5′-TCATATGTATATCTCCTTCTTAAATCTAGAAGTGGTGGCCATTATAGGGAGTTATTCCG-3′
	Seq-GFP-FW	5′-ATAAGTGCGGCGACGATAGTCAT -3′
prrnBP1-psp-TP	psp-vector-FW	5′AATAACTCCCTATAATGCGCCACCACTATGGGTTTGAAGATGAAGAAAAGATCAG-3′
	psp-vector-RV	5′-AACGCTGTAAAACGGGCAATAATTGTTCAGCCTCTGAATGGCGGGAGTATGAAAA-3′
	rrnB2-insert-FW	5′-CCATACTTTTCATACTCCCGCCATTCAGAGGCTGAACAATTATTG CCCGTTTTAC-3′
	rrnB2-insert-RV	5′-GCCTGATCTTTTCTTCATCTTCAAACCCATAGTGGTGGCGCATTATAGGG-3′
	Seq-psp-FW	5′-AAAATCGAGATAACCGTTGGCC-3′

* FW: forward primer; RV: reverse primer. ** The underlined nucleotides are the homologous sequences for the assembly.