Table 1.
Formulation of a Dulbecco’s modified Eagle medium (DMEM) with high glucose.
| Inorganic salts/buffers: CaCl2: 0.2 g/L, Fe(NO3)3 × 9 H2O: 0.0001 g/L, MgSO4: 0.09767 g/L, KCl: 0.4 g/L, NaHCO3: 3.7 g/L, NaCl: 6.4 g/L, NaH2PO4: 0.109 g/L |
| Amino acids: L-Arginine × HCl: 0.084 g/L, L-Glutamine: 0.584 g/L 1, Glycine: 0.03 g/L, L-Histidine × HCl × H2O: 0.042 g/L, L-Isoleucine: 0.105 g/L, L-Leucine: 0.105 g/L, L-Lysine × HCl: 1.46 g/L, L-Phenylalanine: 0.066 g/L, L-Serine: 0.042 g/L, L-Threonine: 0.095 g/L, L-Tryptophan: 0.016 g/L, L-Tyrosine × 2 Na × 2 H2O: 0.12037 g/L, L-Valine: 0.094 g/L |
| Vitamins: Choline chloride: 0.004 g/L, Folic acid: 0.004 g/L, myo-inositol: 0.0072 g/L, Niacinamide: 0.004 g/L, D-Pantothenic acid (hemicalcium): 0.004 g/L, Pyridoxal hydrochloride: 0.004 g/L, Riboflavin: 0.0004 g/L, Thiamine × HCl: 0.004 g/L |
| Others: D-Glucose: 4.5 g/L 2, Phenol red × Na: 0.0159 g/L 3, Pyruvic acid × Na: 0.11 g/L |
1 In some formulations, L-Glutamine should be freshly added, 2 the concentration of D-Glucose in DMEM with low glucose is 1 g/L, 3 Phenol red has a weak estrogenic activity [20] and is therefore omitted in some DMEM formulations.