Skip to main content
. 2023 Feb 22;12(5):697. doi: 10.3390/cells12050697

Figure 3.

Figure 3

Temporal kinetics of NF-κB and ERK1/2 phosphorylation. (a–e) Opto-TLR4-LOV LECs were (a) continuously stimulated with LPS (100 ng/mL), (b) exposed to blue light (470 nm) for 30 min and then put into dark, or (c) continuously exposed to blue light (470 nm). (d) Opto-TLR4-LOV HUVECs were continuously treated with LPS (100 ng/mL) or illuminated with blue light (470 nm) for 24 h. (e) Opto-TLR4 ΔECD-LOV LECs were exposed to blue light (470 nm) for 24 h. Western blots of whole-cell extracts were probed with antibodies against phospho-p65 (p-p65), p65, phospho-extracellular-signal-regulated kinase 1/2 (p-ERK1/2), ERK1/2, and vinculin as a loading control.