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. 2023 Feb 27;12(5):756. doi: 10.3390/cells12050756

Figure 8.

Figure 8

Tnp2 mRNA–miRNA122a interaction. Modulation of translation of Tnp2 depends on the presence of Tnp2 3′ UTR region. (A) Schematic representation of the psiCheck2 reporter gene carrying the Tnp2 coding sequence with or without 3′ UTR. (B) Relative luciferase activity in COS-1 cells co-transfected with Tnp2 with 3′UTR reporter constructs (or Tnp2 coding region without 3′UTR) and miR122a mimic and/or miR122a inhibitor or negative control. Asterisks (*) indicate statistically significant change between psi-Tnp2 and psi-Tnp2 + miR122a mimic group (Student’s t-test; p < 0.05). All the data represent means ± SEM from three independent experiments, with each sample running in triplicates.