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. 2023 Mar 6;15(5):1620. doi: 10.3390/cancers15051620

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Macrophages tend to be M2 polarized after high-dose IR. Flow-cytometric profile of macrophages in tumor tissues of subcutaneously implanted Hela (A) and SiHa (B) mouse models of cervical cancer. Tumor tissues were isolated from each group (n = 8) on the 3rd or 8th day after 2 Gy or 8 Gy IR. Macrophages were gated as CD45+/GR-1–/CD11b+/F4/80+ immune cells. CD11c+ M1 and CD206+ M2 were gated on macrophages. Stained cells were subjected to flow cytometry. The data were analyzed using one-way ANOVA. ** p < 0.01, *** p < 0.001.