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. 2001 Dec;21(24):8521–8532. doi: 10.1128/MCB.21.24.8521-8532.2001

FIG. 5.

FIG. 5

FIG. 5

FIG. 5

(a) Subcellular localization of p53, p53ΔI, and p53K6R in the presence of cotransfected MDM2. Costaining with DAPI shows localization of the nucleus. Identical results were obtained with H1299 cells (data not shown). (b) Graph summarizing three independent experiments with U2OS cells, showing the proportion of cells expressing cytoplasmic p53 following transfection with GFP-p53, GFP-p53ΔI, GFP-p53K6R, and MDM2 as indicated. (c) Heterokaryon assays between U2OS cells transfected with p53, p53K6R, or p53ΔI and p53/MDM2 −/− MEFs. After cell fusion, p53 protein was detected in human and mouse nuclei (white arrow).