FIG. 1.

(a) Schematic representation of the GAGA isoform constructs used in this study. Both transgenes are driven by the hsp83 promoter. (b) Western blot showing expression of the hsp83:GAGA-519 and hsp83:GAGA-581 transgenes. Lanes: 1, embryos from a cross of w¹ males and females; 2, embryos from a cross of 4Xhsp83:GAGA-519; Trl^81.1/Trl^109.2 males and females; 3, embryos from a cross of 4Xhsp83:GAGA-581; Trl^R67/Trl^109.2 males and females. The first three lanes (left half of the gel) were probed with the antibody against GAGA-519 (α-GAGA-519). The weak band corresponding to the GAGA-519 protein in lane 1 is indicated with a star. The right half of the gel was probed with the GAGA-581 antibody (α-GAGA-581). This antibody recognizes a nonspecific band that appears in all three lanes. The band corresponding to the GAGA-581 protein is indicated with a star. α-Snf antibody was used as a loading control.