FIG. 3.

Rescue of Trl phenotypes by GAGA-519 and GAGA-581. (a) Rescue of abdominal transformations in males. Siblings with (white columns) and without (black columns) a transgene were compared. The number above each bar represents the total number of males examined. Values for Trl^13C-homozygous males shown represent averages between the crosses involving different lines of the same transgene. Rescue by GAGA-519 was highly significant, with all P values less than 10⁻⁸, calculated by Fisher's exact test. (b) Rescue of female fertility. Error bars represent 95% confidence intervals. The numbers of eggs laid by single females were measured as described in Materials and Methods. In the left panel, the gray column corresponds to the wild-type females, the black column corresponds to the Trl^13C-homozygous females, and the white columns correspond to Trl^13C-homozygous females carrying the transgenes. (c) Rescue of maternal effect lethality. Error bars represent 95% confidence intervals. The hatch rate was determined after 48 h at 22°C, as described in Materials and Methods. The colors of the columns correspond to the same genotypes as for panel b.