FIG. 6.
Effects of hsp83:GAGA-LacZ on viability and Trl loss of function phenotypes. (a) A schematic representation of the LacZ fusion protein. Expression was driven with the hsp83 promoter. (b) One copy of hsp83:GAGA-LacZ rescues viability of Trl13C/TrlR67 flies. Genotypes represented are as follows: 1, Trl13C/TrlR67; 2, 1Xhsp83:GAGA-LacZ #6; Trl13C/TrlR67; 3, 1Xhsp83:GAGA-LacZ #8; Trl13C/TrlR67. Siblings with and without the transgene were compared. The viability of Trl13C/TrlR67 represents an average between two crosses involving different lines of the transgene. All P values were smaller than 10−4, obtained by the χ2 test. (c) Rescue of abdominal transformations in males. The genotypes of males examined were as follows: bar 1, Trl13C/Trl13C; bar 2, 1Xhsp83: GAGA-LacZ #6; Trl13C/Trl13C; bar 3, 1Xhsp83: GAGA-LacZ #8; Trl13C/Trl13C. Siblings with and without the transgene were compared. The number above each bar represents the total number of males examined. Values for Trl13C-homozygous males shown represent averages between two crosses involving different lines of the transgene. (d) Rescue of maternal-effect lethality. The genotypes of females shown are as follows: bar 1, w1; bar 2, Trl13C/Trl13C; bar 3, 1Xhsp83:GAGA-LacZ #6; Trl13C/Trl13C. Error bars represent 95% confidence intervals. The hatch rate was determined after 48 h at 22°C, as described in Materials and Methods. (e) Lethal effects of the hsp83:GAGA-LacZ transgene in one copy (black bars) and two copies (white bars). The number above each bar indicates the total number of flies scored in a cross. The viability of flies with one copy of the transgene was compared to that of w1 siblings. The viability of flies carrying two copies of the transgene was compared to that of heterozygous siblings. Effects in two copies were assayed in trans-heterozygotes to avoid site of insertion effects. All P values for two-copy lethality were less than 10−11, calculated using the χ2 test.