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. 2023 Jan 13;15(3):mfad003. doi: 10.1093/mtomcs/mfad003

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© The Author(s) 2023. Published by Oxford University Press.

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Fig. 3. — Ni,Zn-HypA titration of H102K-UreE₂. (A) Heat response for injections of 0.45 mM Ni,Zn-HypA into 30 μM H102K-UreE₂. (B) Integrated heat data of the titration as a function of Ni,Zn-HypA/H102K-UreE₂ molar ratio. The continuous line represents the best fit (GoF = 68.4%) obtained with a model involving a global fit according to Eqn. 5. (C) Concentration distribution of the different species occurring during the titration described in (B), calculated using the thermodynamic parameters obtained from the fit and the binding model described by Eqn. 5.