Skip to main content
. 2023 Jan 13;15(3):mfad003. doi: 10.1093/mtomcs/mfad003

Fig. 4.

Fig. 4.

Ni,Zn-HypA titration of H152A-UreE2. (A) Heat response for injections of 0.45 mM Ni,Zn-HypA into 30 μM H152A-UreE2. (B) Integrated heat data of the titration as a function of Ni,Zn-HypA/H152A-UreE2 molar ratio. The continuous line represents the best fit (GoF = 58.4%) obtained with a model involving a global fit according to Eqn. 7. (C) Concentration distribution of the different species occurring during the titration described in B), calculated using the thermodynamic parameters obtained from the fit and the binding model described in Eqn. 7.