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. 2023 Feb 21;24(5):4295. doi: 10.3390/ijms24054295

Figure 2.

Figure 2

E-juice treatment increased TRAIL, TNF-α release and cytotoxicity in human precision-cut lung slices (PCLS) infected with influenza A virus (IAV). PCLS were treated with IAV and E-juice (EJ) for 24, 48, and 72 h. (A) TRAIL release (72 h) in supernatants analyzed by western blotting. (B) TNF-α release (72 h) in supernatants analyzed by ELISA. (C–E) Cytotoxicity was analyzed by performing the LDH assay. Each data point (colored dot) represents the average of 3 replicates from each respective individual donor (n = 5 donors). Horizontal lines indicate mean ± SEM. Paired t-test was used to analyze the data.