FIG. 2.

Absence of PPEF-2 protein in PPEF-2^−/− mice and PPEF-1 RNA in 2×KO mice. (A) Immunoblot analysis of 20 μg of detergent-solubilized retina protein from wild-type, PPEF-2^+/−, and PPEF-2^−/− mice. Molecular mass markers are indicated in kilodaltons. (B) RT-PCR analysis of brain stem and eye RNA from wild-type (WT) and 2×KO (KO) mice using PPEF-1 specific primers in exons 5 and 6. G3PDH, GAPDH; neg, negative control. (C and D) PPEF-2 immunoreactivity in photoreceptor inner segments and synaptic terminals of wild-type mice (C) and lack of PPEF-2 immunoreactivity in PPEF-2^−/− mice (D). OS, photoreceptor outer segments; IS, photoreceptor inner segments; ONL, outer nuclear layer; and OPL, outer plexiform layer. Scale bars in panels C and D correspond to 25 μm.