Figure 6.

Wound fluid bacterial killing assay based on colony forming units (CFU/mL). (A) PaTx-II effectively inhibited the growth of S. aureus and completely cleared the growth of bacteria after 14 days versus the control. Graphical representation of wound fluid (WF) shows the percentage inhibition of bactericidal clearance after 24 h incubation at 37 °C. (B) Collagen accumulation and breaking strength of wounds were compared. New collagen accumulation was found in excised wounds and control skin. The difference between the curves was calculated, representing collagen accumulation. Statistical comparisons were performed with Prism 4.0 Software (Graph-Pad), using a two-tailed Student’s t-test for the comparison of two data sets, and ANOVA for multiple comparisons. Statistically significant differences from controls were observed for PaTx-II and FAO (p < 0.01; **).