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. 2023 Feb 24;24(5):4486. doi: 10.3390/ijms24054486

Figure 2.

Figure 2

Effects of EF-2001 on lipid accumulation in FL83B hepatocytes. (A) Effects of EF-2001 on lipid accumulation induced FL83B hepatocytes with ORO staining. FL83B hepatocyte lipid accumulation induced by 0.5 mM oleic acid (OA) for 48 h. FL83B hepatocyte media was changed for new serum free media in the absence or presence of EF-2001 (25–250 μg/mL) at 24 h. Lipid accumulation of FL83B hepatocyte was observed by ORO staining. (B) Effects of EF-2001 on the relative lipid accumulation of FL83B hepatocyte. ORO stained FL83Bs were eluted with 100% isopropanol, and optical density (O.D.) was measured at 490 nm. Data are represented as mean ± SEM. (n = 4); ** p < 0.01, and *** p < 0.001 vs. control.