Figure 3.

Effect of EF-2001 on confocal microscopy detection of neutral lipid droplet by BODIPY 493/507 in FL83B hepatocyte. FL83B hepatocyte lipid accumulation induced by 0.5 mM oleic acid (OA) for 48 h. FL83B hepatocyte media was changed in the presence of various doses of EF-2001 (0, 25, 50, 100, and 250 μg/mL) for 24 h. Neutral lipid was marked by BODIPY 493/503 (green), and nuclei were stained with 4′,6-diamidino-2-phenylindole (blue). The stained neutral lipid was represented with confocal microscopy at ×200 magnification.