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. 2023 Feb 24;24(5):4486. doi: 10.3390/ijms24054486

Figure 6.

Figure 6

Effects of EF-2001 on AMPK signaling pathways with 5-aminoimidazole-4-carboxamide-1-β-D-ribofuranoside (AICAR) and compound C in FL83B hepatocytes. The AMPK signaling pathway proteins were detected by Western blot. (A) FL83Bs were treated with 0.5 mM oleic acid (OA) for 48 h, followed by EF-2001 (250 μg/mL) with or without AMPK enhancer (AICAR) for 24 h. The relative protein expression levels of p-AMPK (B), p-ACC (C), SREBP-1C (D), and ATGL (E) are shown in the graphs. The fold expression levels were measured relative to the expression of β-actin. Three independent experiments were analyzed, and the fold expression levels were measured relative to the expression of β-actin. Data are presented as mean ± SEM; * p < 0.05 and ** p < 0.05 compared with OA group.