Figure 8.

Effects of EF-2001 on lipase and AMPK signaling pathways in HFD-induced fatty liver. Male rats were divided into SD or HFD groups and orally administered refined water, 3 mg/kg or 30 mg/kg EF-2001 for 6 weeks (n = 6 rats/group). Lipase and AMPK signaling pathway were detected by immunoblotting. (A) The protein levels of p-AMPK, SREBP-1C, p-ACC, ATGL, and MGL were standardized depending to the amount of β-actin. The relative protein expression levels of p-AMPK (B), SREBP-1C (C), p-ACC (D), ATGL (E), and MGL (F) are shown in the graphs. Data are represented as the mean ± SEM. (n = 6); * p < 0.05 vs. HFD-fed rats alone without EF-2001.