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. 2023 Feb 28;24(5):4675. doi: 10.3390/ijms24054675

Figure 4.

Figure 4

VitD/cisplatin combinations synergistically enhance the cisplatin-mediated killing of HNC tumor cells. (a) Cotreatment of VitD/cisplatin synergistically triggered the cell death of HNCUM-02T, FaDu, Pica, and SCC-4 cells compared to cisplatin alone. Cells were seeded in the presence of 100 nM VitD, and after 24 h, the cells were treated with 15–20 µM cisplatin with or without VitD (100 nM). The viability of the untreated control was set to 1. (b–e) Chou–Talalay dose–effect curves and calculation of the combination index (CI) demonstrate the synergistic effect of VitD/cisplatin combination treatments in HNCUM-02T, FaDu, Pica cells, and SCC-4, respectively. ** p < 0.01 and **** p < 0.0001.