Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

[Preprint]. 2023 Feb 14:2023.02.13.527055. [Version 1] doi: 10.1101/2023.02.13.527055

PMC10002642.1; 2023 Feb 14
PMC10002642.2; 2024 Jul 31

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License, which allows reusers to distribute, remix, adapt, and build upon the material in any medium or format for noncommercial purposes only, and only so long as attribution is given to the creator.

PMC Copyright notice

SMART-seq approach on manually collected M- and S-cone samples. a) Cones after dissociation. P: pedicle, CB: cell body, IS: inner segment, OS: outer segment. b-c) Fluorescently labeled live cones after antibody incubation under bright field (b) and excitation light (c) conditions. Labeled cone (arrowhead) was categorized as S-cone, while cells with cone morphology but without label (arrows) were categorized as M-cones. d)-e) Differentially expressed genes in S-and M-cone photoreceptors. d) Volcano plot highlighting significant genes enriched in M-cones (logFC < 0) and S-cones (logFC > 0). e) Heat map showing expression of significant differentially expressed genes in all samples. f) Validation of cone subtype-specific gene expression of Nrxn3. Flat-mounted ground squirrel retina after fluorescent in situ hybridization (FISH) with Nrxn3-probe, followed by S-opsin antibody-staining. Magenta: anti-S-opsin antibody. Green: Nrxn3 FISH probe. Scale bar: 10 μm.