Fig. 6: Modulating Serpinb2⁺ neuron activity can overcome leptin effect and alter bodyweight.

a, Diagram showing bilateral cannula implantation in LH for leptin delivery (left). CNO delivery was achieved via i.p. injection. Results of total food consumption in 3 hours by fasted mice with different doses of leptin administration. Scale bar: 500 μm.

b, Same as panel A except food consumption is quantified under different conditions with or without Serpinb2⁺ neuron activation in the presence or absence of 1 μg of leptin delivery. ***P ≤ 0.001, **P ≤ 0.01, *P ≤ 0.05; ns, P > 0.05, unpaired t-test.

c, FISH and quantification verify Serpinb2⁺ neuron ablation after AAV-DIO-taCasp3 injection. Scale bar: 100 μm. ***P ≤ 0.001, unpaired t-test.

d, Serpinb2⁺ neuron ablation decreased 3 hours total food consumption by mice. ***P ≤ 0.001, unpaired t-test.

e, Serpinb2⁺ neuron ablation has a long-time effect on bodyweight loss. ***P ≤ 0.001, **P ≤ 0.01, *P ≤ 0.05; ns, P > 0.05, unpaired t-test.

Data are presented as means ± SEM.