Table 2.

C1r binding and inhibitory CP ELISA data.

	FbpC-C	FbpC-C R248A	FbpC-C H252A
SPR: Zymogen C1r KD (nM)	1.2 ± 0.034	13 ± 6.6	N.D.
ka (1/Ms)	4.4×104 ± 1.2×104	6.9×104 ± 7.0×104	N.D.
kd (1/s)	5.4×10−5 ± 1.4×10−5	6.3×10−4 ± 3.6×10−4	N.D.
SPR: Active C1r KD (nM)	0.3 ± 0.014	0.19 ± 0.048	2.4 ± 0.12
ka (1/Ms)	4.8×104 ± 0.048×104	4.5×104 ± 0.52×104	5.1×104 ± 0.63×104
kd (1/s)	1.4×10−5 ± 0.076×10−5	9.4×10−6 ± 3.1×10−6	1.2×10−4 ± 0.19×10−4
CP ELISA IC50 (nM)	8.6 (7.5–9.8)	23 (20–26)	300 (240–400)

N.D. stands for not determined.