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[Preprint]. 2023 Mar 1:2023.02.28.530493. [Version 1] doi: 10.1101/2023.02.28.530493

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Fig. 1 | — Schematic showing three broad steps of SaGA: 1) Preparation, 2) Selection and Isolation, and 3) Analysis. SaGA can be applied to a variety of cell conditions, such as non-adherent, 3-dimensional (3D), and 2-dimensional (2D), for selection, isolation, and analysis of live subpopulations within a parental population. Cells stably expressing a photoconvertible tag can be precisely photoconverted (from green to red) based upon live, user-defined, phenotypic criteria. These red photoconverted cells are then isolated utilizing fluorescence activated cell sorting (FACS) for multi-omic analysis and/or cell cultivation for long-term in vitro and in vivo analyses. Created with Biorender.com.