Fig. 4 |. Example photoconversion in different cell culture conditions.

a. Cells stably expressing a photoconvertible tag (ex: H2B-Dendra2, Pal-Dendra2) can be prepared under non-adherent, 3D, or 2D experimental conditions which illicit distinct and imageable cellular response for photoconversion. Non-adherent conditions were performed with RPMI8226 myeloma cells; H1299 lung cancer cells were used for all other conditions. Scale bar, 50 μm. b, c. Integrated density (relative fluorescence units) quantification of 6 or more cells pre- and post-photoconversion in the green (b) and red (c) channels, emission peaks, 507 nm, and 573 nm, respectively. d, e. Quantification of integrated density percent change of 6 or more cells pre- and post- photoconversion in the green (d) and red (e) channels.