Table 1 |.

Example downstream applications of SaGA-isolated subpopulations

Experimental approach		Application	Potential outcome
Immediate isolation	In vitro	Cell lysis for immediate contents extraction (i.e., protein, RNA, DNA, and ribosomes)	Targeted transient expression profiling via immunoblotting, qPCR, etc.
			Unbiased transient expression profiling via ATACseq, RNAseq, Riboseq, etc.
Long-term cultivation in vitro	In vitro	Cell behavior, signaling, etc.	Stable phenotype identification, stable subpopulation generation, determination of cooperative phenotype between subpopulations, targeted expression profiling and unbiased multi-omic analysis.
	In vivo	Introduction to model organism	Stable phenotype identification, determination of cooperative phenotype between subpopulations, targeted expression profiling and unbiased multi-omic analysis.