Skip to main content
[Preprint]. 2023 Mar 2:2023.03.01.530729. [Version 1] doi: 10.1101/2023.03.01.530729

Figure 1. ∆oprF forms less biofilm in TSB than in LB, due to lower sodium chloride concentration and presence of glucose.

Figure 1.

(A) 24-hour static microtiter biofilm assays of P. aeruginosa PAO1 (WT, black), ∆pslD (blue), ∆oprF (red), and a ∆oprF attTn7::PBAD-orpF restoration strain (∆oprF + oprF) without (white) and with (gray) 0.5% arabinose (ara) in the indicated media. Error bars, SEM (N = 3); asterisk over error bar, statistically different from WT in the same medium (p < 0.05; two-way ANOVA with post hoc Bonferroni). Statistical difference between ∆oprF strains in different media are indicated by a bar and asterisk. (B and C) Biofilm formation of ∆oprF strain in variations of TSB and LB: unaltered, altered NaCl concentrations, altered glucose concentrations, and altered K2HPO4 concentrations (left to right). Biofilm formation is normalized to WT in each respective medium. Dashed line, normalized amount of WT biofilm formation in each medium; error bars, SEM (N = 3); asterisk over error bar, statistically different from ∆oprF in the original medium (p < 0.05; two-way ANOVA with post hoc Bonferroni). See Figure S4 and Tables S2-S3 for full comparisons.