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[Preprint]. 2023 Mar 2:2023.03.01.530729. [Version 1] doi: 10.1101/2023.03.01.530729

Figure 2. OprF affects late biofilm maturation in TSB.

Figure 2.

(A) 1-hour static microtiter biofilm assays were performed in TSB with PAO1 (WT, black), ∆pslD (blue), ∆oprF (red), and a ∆oprF attTn7::PBAD-orpF restoration strain (∆oprF + oprF) with (white) and without (gray) 0.5% arabinose (ara). (B) 4-hour, (C) 8-hour, (D) 16-hour, and (E) 24-hour assays were performed with the same strains and media. (F) Static ∆oprF biofilm formation relative to WT (dashed line) at respective time points is represented. Biofilm formation is normalized to WT in each respective medium. Error bars, SEM (N = 3); letters, statistical groupings (p < 0.01; one-way ANOVA with post hoc Tukey HSD); asterisk, statistically different from WT at the same time point.