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[Preprint]. 2023 Mar 2:rs.3.rs-2597043. [Version 1] doi: 10.21203/rs.3.rs-2597043/v1

Figure 2. Adult neurogenesis is decreased in subgranular zone of mouse hippocampus in Tdrd3-null mice, especially for the type II subtype neural stem cells.

Figure 2

a-c Images (a) and their Quantifications (b-c) to show that proliferation of aNSCs in Tdrd3-null mice were reduced significantly compared to WT mice. This is indicated by decreased density of BrdU+ cells (b) and BrdU+Ki67+ cells (c) in BrdU pulsed-labeling experiments. Color codes in (a): Red, BrdU; White, Ki67; Blue, DAPI. Mouse numbers: WT=4, Tdrd3-null=4, with 7–8 slices/mouse. d A scheme of lineage-specific markers during hippocampal neural stem cell development. The cells at different developmental stages are shown at the top, whereas their markers are shown at the bottom. e-h Images (e-f) and their Quantifications (g-h) to show that the type II but not type I aNSCs in SGZ are compromised in Tdrd3-null mice. This is revealed by decreased BrdU+Sox2+GFAP− cell density (e, f, h) but unaltered BrdU+Sox2+GFAP+ cell density (g) (Red, BrdU; Green, GFAP; White, Sox2; Blue, DAPI). Number of mice used: WT=4, Tdrd3-null =4, with 4–6 slices/mouse. Data are presented as mean values + SD. Two-tail Student’s t test was performed for (b), (c), (g), (h). p-values < 0.001, and 0.0001 are marked as: ***, and ****; p-value > 0.5 is marked as ns.