Table 2.
List of used growth factors, inhibitors and small molecules for DA-neuron differentiation.
| Substance | Company | Concentration | Days |
|---|---|---|---|
| LDN193189 | Sigma-Aldrich, Darmstadt, Germany | 100 nM | 0–11 |
| SB431542 | Abcam, Cambridge, UK | 10 µM | 0–5 |
| Purmorphamine | Tocris, Ellisville, MO, USA | 2 µM | 1–7 |
| SHH C25II | PeproTech, Cranbury, NJ, USA | 100 ng/mL | 1–7 |
| FGF8b | PeproTech, Cranbury, NJ, USA | 100 ng/mL | 1–7 |
| CHIR99021 | Sigma-Aldrich, Darmstadt, Germany | 3 µM | 3–13 |
| BDNF | PeproTech, Cranbury, NJ, USA | 20 ng/mL | 13–to … |
| GDNF | PeproTech, Cranbury, NJ, USA | 20 ng/mL | 13–to … |
| TGFb3 | PeproTech, Cranbury, NJ, USA | 1 ng/mL | 13–to … |
| dbcAMP | PeproTech, Cranbury, NJ, USA | 0.5 mM | 13–to … |
| Compound E | Millipore, Burlington, VT, USA | 0.1 µM | terminal stage |