Table 3.
List of used antibodies.
| Antibodies | Company | Cat. Ref. | Raised/Type | Dilution |
|---|---|---|---|---|
| Primary antibodies | ||||
| Anti-OCT4 | Abcam, Cambridge, UK | ab18976 | IgG rabbit polyclonal | 1:200 |
| Anti-SOX2 | Cell Signaling, Danvers, MA, USA | 3579 | IgG rabbit polyclonal | 1:500 |
| Anti-SSEA4 | Abcam, Cambridge, UK | ab16287 | IgG3 mouse monoclonal | 1:200 |
| Anti-TRA-1-60 | Abcam, Cambridge, UK | ab16288 | IgM mouse monoclonal | 1:200 |
| Anti-LRTM1 | EpiGentek, Farmingdale, NY, USA | A67852 | IgG rabbit polyclonal | 1:100 |
| Anti-PAX6 | Santa Cruz Biotechnology, Dallas, TX, USA | sc-81649 | IgM mouse monoclonal | 1:50 |
| Anti-SOX1 | R&D systems, Minneapolis, MN, USA | AF3369 | IgG goat polyclonal | 1:200 |
| Anti-OTX2 | R&D systems, Minneapolis, MN, USA | AF1979 | IgG goat polyclonal | 1:400 |
| Anti-TH | Millipore, Burlington, VT, USA | AB152 | IgG rabbit polyclonal | 1:400 |
| Anti-LMX1A | Abcam, Cambridge, UK | ab139726 | IgG rabbit polyclonal | 1:100 |
| Anti-TUJ1 | Covance, Princeton, NJ, USA | MMS-435P | IgG2a mouse monoclonal | 1:1000 |
| Secondary antibodies | ||||
| Alexa Fluor 488 goat anti-rabbit IgG (H+L) | Thermo Fisher Scientific, Waltham, MA, USA | A11008 | Made in goat | 1:400 |
| Alexa Fluor 568 goat anti-rabbit IgG (H+L) | Thermo Fisher Scientific, Waltham, MA, USA | A11011 | Made in goat | 1:400 |
| Alexa Fluor 488 goat anti-mouse IgG (H+L) | Thermo Fisher Scientific, Waltham, MA, USA | A11029 | Made in goat | 1:400 |
| Alexa Fluor 568 goat anti-mouse IgG (H+L) | Thermo Fisher Scientific, Waltham, MA, USA | A11031 | Made in goat | 1:400 |
| Alexa Fluor 488 goat anti-mouse IgG2a | Thermo Fisher Scientific, Waltham, MA, USA | A21131 | Made in goat | 1:400 |
| Alexa Fluor 488 donkey anti-goat IgG (H+L) | Thermo Fisher Scientific, Waltham, MA, USA | A11055 | Made in donkey | 1:400 |
| Alexa Fluor 594 rabbit anti-mouse IgG (H+L) | Thermo Fisher Scientific, Waltham, MA, USA | A11062 | Made in rabbit | 1:400 |