Table 4.
Target | Forward/Reverse Primer (5′-3′) | |
---|---|---|
House-keeping gene (RT-qPCR) | beta-2-microglobulin | TAGCTGTGCTCGCGCTACT/ TCTCTGCTGGATGACGTGAG |
TFRC | AGCAGTTGGCTGTTGTACCTCTC/ GTCGCTGGTCAGTTCGTGATT |
|
ACTB | GTGCGTGACATTAAGGAGAAG/ GAAGGAAGGCTGGAAGAGTG |
|
Gene expression analysis (RT-qPCR) [52] |
GBA | TCCAGGTCGTTCTTCTGACT/ ATTGGGTGCGTAACTTTGTC |
DA-specific gene expression (RT-qPCR) | TH | AAAGTGTCAGAGCTGGACAAG/ GAAGGCGATCTCAGCAATCA |
LMX1A | CTTGCATTCTTGCTCTCTTTGG/ CAGGAGTCTGGGCTTTACATT |
|
NURR1 | CAGAGCTACAGTTACCACTCTTC/ TGGTGAGGTCCATGCTAAAC |