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. 2023 Feb 28;24(5):4668. doi: 10.3390/ijms24054668

Figure 2.

Figure 2

Collagen content and expression are increased in bleomycin-treated skin from MKP-1-deficient mice. (A) Representative images of Masson’s trichrome-stained sections of control skin and skin treated with bleomycin from wild-type (WT) and MKP-1-deficient (MKP-1−/−) mice are shown. Original magnification 10×, magnification in insets 20×, scale bar 200 µm. (B) Collagen content (% of collagen positive area in dermis was measured using Image J program. (C,D) Collagen 1A1 (Col1A1; (C)) and collagen 3A1 (Col3A1; (D)) mRNA expression in control skin and in skin treated with bleomycin from wild-type (WT) and MKP-1-deficient (MKP-1−/−) mice was measured by quantitative RT-PCR analysis and normalized against GAPDH mRNA. Col1A1 and Col3A1 levels in WT control skin was set as 1, and the other values are given in relation to that value. Bars represent mean + SEM; n = 8 in the control groups and n = 6 in the bleomycin-treated groups. Statistical significance of the results was calculated by two-way ANOVA followed by Tukey’s multiple comparisons test.