Fig. 3.

OXPHOS complex IV decreases with age. (A) Equal amounts of isolated mitochondria from both the cohorts subjected to mtDNA isolation and subsequent qPCR to test the different mt-genes in order to estimate the relative mtDNA amounts (mean±s.e.m., n=4). (B) Isolated mitochondria from both the cohorts subjected to Tris Tricine-SDS-Page and immunoblotting to check for OXPHOS (n=3). (C) Area under the curves were calculate with ImageJ and were normalized to Vdac (mean±s.e.m., * denotes P<0.05 from two-tailed unpaired Students t-test, n=3) (D) Complex IV activity was measured using activity assay microplate kit (Abcam). Complex IV activity was normalized to activity of a young mice samples and the mean±s.e.m. is shown (* denotes P<0.05 from two tailed unpaired t-test, n=3).