Fig. 4.

OXPHOS complex IV decreases with age. (A) Experimental overview of the purification of mitochondrial samples isolated from the livers of the experimental cohorts, RNA isolation, library preparation and Nanopore sequencing. (B) Representative image of mt-Rnr2 sequencing. Bam alignment file visualization with Geneious Prime shows the reference sequence highlighted in yellow and the consensus is marked in blue. Blue box shows a magnified view of the alignment in the region where the poly dT primer anneals during the library preparation.