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. 2023 Mar 1;24(5):4756. doi: 10.3390/ijms24054756

Figure 4.

Figure 4

SMARCA4 is a target gene of miR-199a-5p in OSCC cells. (A) The alignment between miR-199a-5p and WT or MUT SMARCA4 3′UTR region. (B,C) SMARCA4 3′UTR containing a miR-199a-5p WT or a MUT target site was cloned into the luciferase reporter vector. These constructs were co-transfected with miR-199a-5p mimics, mimics NC, miR-199a-5p inhibitor, or inhibitor NC into 293T cells. Luciferase activity was measured after 48 h. (D,E) The transfection efficiency of miR-199a-5p mimics, mimics NC, miR-199a-5p inhibitor or inhibitor NC in OSCC cells. SAS or CAL-27 were transfected with miR-199a-5p mimics, mimics NC, miR-199a-5p inhibitor, or inhibitor NC for 48 h. The expression level of miR-199a-5p in OSCC cells was measured by qRT-PCR. (F,G) The effect of miR-199a-5p on SMARCA4 mRNA expression level in OSCC cells. SAS or CAL-27 cells were transfected with miR-199a-5p mimics (F) or miR-199a-5p inhibitors for 48 h (G). The mRNA expression level of SMARCA4 in OSCC cells was measured by qRT-PCR. (H,I) The effect of miR-199a-5p on SMARCA4 protein expression level in OSCC cells. SAS or CAL-27 cells were transfected with miR-199a-5p mimics (H) or miR-199a-5p inhibitors for 48 h (I). SMARCA4 protein level was measured by Western blot analysis. GAPDH was used as a loading control. Data are expressed as the mean ± SEM from triplicate experiments. * p < 0.05, ** p < 0.01, **** p < 0.001, comparison with the corresponding control.