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. 2023 Mar 1;24(5):4756. doi: 10.3390/ijms24054756

Figure 5.

Figure 5

Effect of miR-199a-5p on OSCC cells EMT and migration. (A) Comparison of miR-199a-5p mRNA levels in SAS, CAL-27, and HaCaT. The miR-199a-5p mRNA level was detected by qRT-PCR. (B) The effect of miR-199a-5p mimics on OSCC cell E-cadherin and vimentin protein expression levels. SAS and CAL-27 cells were transfected with miR-199a-5p mimics 48h, and the E-cadherin or vimentin protein expression levels were measured by Western blot analysis. (C) The protein bands were analyzed by Image J. (D–G) The effect of miR-199a-5p mimics or miR-199a-5p inhibitor on OSCC cell migration. SAS cells or CAL-27 cells were transfected with miR-199a-5p mimics or miR-199a-5p inhibitor for the indicated times (6 h for SAS, 30 h for CAL-27), and cell migration was measured by the wound healing assay. Scale bar: 100 µm (D,F). Quantitative analysis was performed using the Image J software (E,G). Data are expressed as the mean ± SEM from triplicate experiments. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.