Figure 1.

Neural SGPL1 ablation triggers glucose degradation in primary cultured astrocytes. (A,B) Protein quantification of phosphofructokinase (PFK), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), pyruvate dehydrogenase (PDH), lactate dehydrogenase (LDH), and isocitrate dehydrogenase (IDH) in astrocytes from control (Ctrl) or SGPL1^fl/fl/Nes (KO) mice, as indicated. (C) IDH activity measurement. (D) Protein quantification of the indicated enzymes following stimulation (+) of control astrocytes with exogenous S1P (10 nM, 24 h), (−) represents without stimulation (E) Quantification of ATP in cultured astrocytes derived from control (Ctrl) and SGPL1^fl/fl/Nes (KO) mice. For all, representative immunoblots are shown with β-actin as loading control. Bars represent means ± SEM, n ≥ 3, unpaired Student’s t-test, one-way ANOVA with Bonferroni multiple comparison test; * p < 0.05, ** p < 0.001, *** p < 0.0001, ns, not significant.