Figure 3.

Modulation of 84 genes in moMΦ stimulated with diverse polarizing factors. Porcine moMΦ were left untreated, or they were stimulated with diverse polarizing factors: IFN-γ + LPS (both at 100 ng/mL), IL-4 (20 ng/mL), IL-10 (20 ng/mL), TGF-β (20 ng/mL), or dexamethasone (“DEXA”, 20 ng/mL). Then, 24 h post-stimulation, macrophage subsets were analyzed using the RT2 Profiler PCR Array for 84 common immune-related genes: (A) The heatmap illustrates fold change expression of these 84 immune-related genes, obtained from macrophage subsets from three diverse pig blood donors. For each macrophage subset, fold change in gene expression was calculated relative to the untreated control (moMΦ). The colors represent the fold change in gene expression compared to the untreated control, with the brightest pink representing the highest value, light grey representing the baseline value (fold change = 1), and blue representing the smallest value; (B) for each macrophage subsets, statistically significantly upregulated genes (fold change > 2, p-value < 0.05) are presented, with the corresponding fold change and p-value; (C) for each macrophage subsets, statistically significantly downregulated genes (fold change > 2, p-value < 0.05) are presented, with the corresponding fold change and p-value.