ASCs-exos facilitate BMSC osteogenesis differentiation in vitro. (A,B) The protein levels of Runx2, collagen I and OCN in BMSCs treated with PBS, AEFS or ASCs-exos were measured by Western blotting. (C) Immunofluorescence staining for collagen I, OCN and Runx2 in different groups. (D) The images of ARS and ALPS of BMSCs after 14 days of incubation with PBS, AEFS or ASCs-exos. (E) The statistical analysis of ARS and ALPS of BMSCs treated with PBS, AEFS or ASCs-exos for 14 days. Scale bars, 100 μm. Data are presented as the mean ± SD, and all experiments were repeated three times. * p < 0.05, ** p < 0.01, *** p < 0.001, # p > 0.05.