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. 2023 Mar 6;24(5):5044. doi: 10.3390/ijms24055044

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Stable transformation of MaMYB113a and MaMYB113b in tobacco. (A) Phenotypic analysis of MaMYB113a and MaMYB113b after transformation into tobacco; CK: empty vector; WT: wild type. (B) PCR analysis of MYB113a/b was performed on tobacco, respectively. Electrophoresis lane 1 and 9: marker; lane 2: MaMYB113A–1; lane 3: MYB113A–2; lane 4: MYB113A–3; lane 5: CK + WT; lane 6: MYB113B–1; lane 7: MYB113B–2; lane 8: MYB113B–3. (C) Relative expression analysis of overexpression of MaMYB113a/b on expression of flower color genes in tobacco leaves by 2^−ΔΔCt. Student’s T test is denoted by *, p < 0.05. (D) Front and back of leaf phenotype of transgenic tobacco and control tobacco. The first row is the leaf front phenotype, and the second row is the leaf back phenotype. Bars: 2 cm.